Research on the safe storage time of 3 kinds of irrigating fluid in incubators at 38℃ in operating room

Abstract

Objective To study the safe storage time of 3 kinds of irrigating fluid in incubators at 38 ℃ in operating room. Methods From March to December 2016, 3 kinds of irrigating liquid, including 500 ml bottled 0.9% Sodium Chloride Solution (A) , 3 000 ml bagged 0.9% Sodium Chloride Solution (B) , and 3 000 ml bagged mannitol (C) were selected. Two hundred and forty bottles (bags) were randomly taken out from each kind of liquid, and divided into 4 groups by random number table method with 60 bottles (bags) in each group. Three of the groups were heated in 38 ℃ incubators for 6 h, 7 d and 30 d, and the last group was set up as the blank control group. According to aseptic procedures, the bacteriological examination, the osmolality, the pH value, the appearance and the peculiar smell of the washing fluid at 6 h, 7 d and 30 d were investigated respectively. Single factor analysis of variance and Least Significant Difference (LSD) t-test were applied in data comparison. Results The results of bacterial cultures of the 3 kinds of liquid were negative, with no appearance change and no odor at 6 h, 7 d and 30 d. The pH and osmolality of all heated groups were all higher than the control group of the same kinds of liquid, and the differences were statistically significant (P 0.05) . Conclusions The results of bacterial culture of all the irrigating fluid heated in 38 ℃ incubators were negative in 30 days, with no apparent change and no peculiar smell. The osmolality and pH changed slightly, but the results were within the normal range. Key words: Operating rooms; Sodium chloride; Mannitol; Irrigating fluid; Heating; pH; Osmolality pressure