Research on the production of feed antibiotic «Kormogrizin» by a mutant strain of <i>Actinomyces griseus</i>

Abstract

This article describes the method of obtaining the food antibiotic kormogrizin using a mutant strain of Actinomyces griseus. It is known that the scope of application of antibiotics in agriculture, medicine and food industry, fodder production is wide. Aerobic, anaerobic and facultative-anaerobic species of actinomycetes were isolated in the course of scientific research work on the production of kormogrizin in laboratory conditions. One of the peculiarities of the microorganisms Actinomyces griseus is that the vegetative cell TB 633 FU undergoes mutagenic changes in the logarithmic phase of growth. When conducting the research, the distance between the Petri dishes was 15 cm, and the BUF-15 lamp was used as an ultraviolet light source. In the research work, the activity of the fodder antibiotic was determined by the diffusion method, and it is also necessary to observe the temperature regime. In the next period of research, they controlled the sensitivity of microorganisms to antibiotics and placed them on paper discs. When the diameter of the inoculation zone of microorganisms was more than 25 mm, the sensitivity of the culture to antibiotics was high, and, accordingly, when the diameter of the inoculation zone was less than 10 mm, the level of sensitivity also decreased. The biological activity of antibiotics is determined by the diffusion method based on the comparison of growth inhibition. The requirements of the melting temperature and the seed medium were taken into account, and a sterile cylinder was used with the help of a stencil. Kormogrizin allows to increase the productivity of farm animals by 10-12%. Emphasis is placed on the main indicators of the antibiotic release process - temperature, duration of cultivation, pH of the medium. The relevance of this article is also high, as it is clear that the production of food antibiotics in laboratory conditions will contribute to the solution of important production and economic problems in the future.