Research on the evaluation indexes of animal model of liver-fire hypertension syndrome

Abstract

Objective: The screening of animal models combined with disease and syndrome is the basis and key to TCM experimental research. According to relevant reports, SHR at 14-18 weeks of age shows a stable type of hypertensive liver-heat syndrome, and the macroscopic appearance and rotation tolerance time of the rat from this experiment. Such as a number of indicators, initially clearly distinguish the relevant microscopic physical and chemical indications of the type of syndrome. Methods: eight 14-week-old SHR were selected as the model group and 8 normal WKY rats were used as the control group. The rats were observed continuously for 5 weeks. Based on the transformation of diagnostic criteria of liver-fire hyperactivity syndrome, macroscopical images of animals were collected. Systolic blood pressure (SBP) and serum angiotensin II(Angll) and noradrenaline (NE) levels were measured by enzyme linked immunosorbent assay (ELISAA) at the age of 18 weeks. Results: compared with the control group, the systolic blood pressure and irritability of the rats in the 14-18 week old model group were obviously increased, while the rotational tolerance time was decreased. The serum Ang I NE increased significantly in the model group at the age of 18 weeks (P<0.05). Conclusion Ang II NE elevation is an important experimental index for evaluating hyperactivity of liver fire in patients with hyperactivity of liver-fire at the age of 14 to 18 weeks.