Research on the Effect of Chemerin on RF/6A Cell Proliferation, Migration and Tube Formation

Abstract

Objective: To investigate the effects of chemerin on the proliferation, migration and tube formation of choroid retinal vascular endothelial cells (RF/6A) in the rhesus monkey in vitro. Methods: In this experimental study, RF/6A cells were randomly divided into 4 groups: a control group, and 3 chemerin groups treated with concentrations of 5, 10 or 20 ng/ml. The cells were cultured in a RPMI-1640 medium. After 24 h and 48 h in the culture, the MTT method was used to detect RF/6A cell proliferation. Cell migration was detected by scratch test, and cell tube formation was detected by matrigel. A one-way analysis of variance was used to compare the data of the multiple groups. Results: MTT results showed that 10 ng/ml and 20 ng/ml concentrations of chemerin significantly promoted cell proliferation compared to the control group (P < 0.05). There was no significant difference between the 5 ng/ml concentration group and the control group. Cell migration results showed that the three different concentrations of chemerin significantly promoted cell migration in a dose-dependent manner. The difference between groups was statistically significant (P < 0.05). The three different concentrations of chemerin significantly promoted cell tube formation in a dose-dependent manner (P < 0.05) in the treated groups compared to the control group. Conclusions: Chemerin can promote the proliferation, migration and tube formation of RF/6A cells, suggesting that chemerin may play an important role in retinal neovascularization. Key words: chemerin; retinopathy; angiogenesis