Research on the development of composition and technology of an emulgel with a probiotic component for the treatment of infectious and inflammatory diseases

Abstract

The aim was conducted research to develop the composition and technology of a new soft preparation under the conventional name "Probioskin" for topical use with a probiotic component in the form of an emulgel.
 Materials and methods of work. The homogeneity was determined by visual inspection of the experimental samples, using a biological microscope XSP-128 ULAB. The rheological (structural and mechanical) properties of the samples were studied using a Rheolab QC rheoviscosimeter (Anton Paar, Austria) with a C-CC27/SS coaxial cylinder system. The Rheolab QC rheometer is equipped with RheoPlus software. Microbiological studies and biotesting on a biological model of infusoria were performed under aseptic conditions of a laminar flow box (biological safety cabinet AC2-4E1 "Esco", Indonesia) of the Department of Biotechnology of the National University of Pharmacy. The identification of lactobacilli and the number of live cells as critical indicators of a preparation containing live microorganisms were performed at each stage of the study. Bacterioscopic control was carried out by examining smears prepared from samples of the dissolved drug and stained by Gram stain. Bacteriological control was performed by inoculation on thick MRS-4 medium and liquid MRS-1 medium. The number of live lactobacilli (when lactobacilli were co-cultured with the drug components at the stages of development and in the finished product) was determined by surface sowing on Petri dishes with thick MRS-4 medium; after 48 h of incubation at (37±1)°C, colonies grown on the surface of the medium were counted and the number of live lactobacilli expressed in CFU was calculated in 1 ml of the drug.
 Results and discussion. Based on the conducted research, the composition of a soft preparation for topical use containing dexpanthenol, lactic acid, lyophilized lactobacillus biomass, propylene glycol, peach oil, polysorbate-80, aristoflex AVC, tocopherol, and purified water was developed. The production process of the developed Probioskin product is carried out according to a standardized scheme and consists 8 stages: preparation of raw materials, preparation of oil concentrate of the probiotic component, preparation of aqueous concentrate of active substances, preparation of gel base, preparation of emulsion, dosing into tubes, packaging of tubes into packs, packaging of packs into group containers.
 Conclusions. On the basis of a complex research, the composition and rational technology of a soft preparation for topical use under the conventional name "Probioskin" in the form of an emulsion was developed.
 Key words: pharmacological research, soft drug, emulgel, probiotics, dermatological diseases