Research on roles played by stromal cell-derived factor 1 and chemokine receptor 4 in diagnosis on proliferation, infiltration and metastasis of cervical cancer

Abstract

Purpose: To understand the influence of Stromal Cell-Derived Factor 1 (SDF-1) and corresponding receptor CXCR4 on proliferation, infiltration and metastasis of cervical cancer HeLa cells. Method: The flow cytometry dyeing and the Western blot method are adopted to detect the expression conditions of CXCR4 in HeLa cells; the MTT method is adopted to detect the influence of SDF-1 and corresponding receptor CXCR4 on proliferation of HeLa cells; the Boyden chamber method is adopted to detect the influence of SDF-1 and corresponding receptor CXCR4 on infiltration and metastasis of HeLa cells. Results: There is high expression on receptor CXCR4, the receptor of SDF-1; after the SDF-1 stimulation, the proliferation ability of HeLa cells is significantly increased (P<0.05), and this effect is dose-dependent; after the knock-down of CXCR4 expression on surface of HeLa cells with RNAi, the proliferation of HeLa cells caused by SDF-1 stimulation is significantly interdicted (P<0.05). SDF-1 may give rise to infiltration and metastasis of HeLa cells. After the knock-down of CXCR4 expression on surface of HeLa cells with RNAi, the infiltration and metastasis of HeLa cells are significantly interdicted (P<0.05). Conclusion: The CXCR4 with high expression on surface of HeLa cells plays a significant role in inducing proliferation, infiltration and metastasis of HeLa cells.