The Effect of Fullerenol Combined with Cisplatin on the Proliferation of Cervical Cancer HeLa Cells

Yuan Niu,Chao Yan

doi:10.4236/jct.2016.73024

Abstract

This study aimed to investigate the effect of fullerenol combined with cisplatin on the proliferation of cervical cancer HeLa cells in order to provide new ideas and laboratory theoretical basis for the clinical treatment of cervical cancer. Cervical cancer cell line HeLa cells in vitro were treated with different concentrations of fullerenol, different concentrations of cisplatin, and different concentrations of fullerenol combined with cisplatin, after 24 h, 48 h, 72 h, microscope changes in cell morphology; MTT assay was used to determine the effect of drugs on the proliferation of HeLa cells. Fullerenol and cisplatin alone can inhibit the proliferation of HeLa cells in a dose-dependent and time-dependent manner; compared with cisplatin alone, different concentrations of fullerenol combined with cisplatin significantly increased the apoptosis rate of HeLa cells (P < 0.05). The inhibition of fullerenol combined with cisplatin on HeLa cells in vitro is more significant, resulting in a stronger anti-cancer effect.

Highlights

Cervical cancer is one of the most common female cancers; female cancer mortality rate was 3.28/100,000
This study aimed to explore the possibility of producing safer and more effective anti-tumor effect with fullerenol combined with cisplatin by observing the changes of fullerenol, cisplatin and fullerenol combined with cisplatin on HeLa cells proliferation and comparing different fullerenol cisplatin and fullerenol cisplatin monotherapy effect, which provided experimental evidence and theoretical basis by using fullerenol in the treatment of cervical cancer
HeLa cells of different concentrations of fullerenol combined with cisplatin showed that cell morphology were similar to the cisplatin group, while cell shrinkage were more apparent and the number of cells reduced obviously (Figures 1-4)

Summary

Introduction

Cervical cancer is one of the most common female cancers; female cancer mortality rate was 3.28/100,000. Fullerenol is a water-soluble fullerene derivative by introducing 18 - 20 hydroxy group at fullerene carbon, has a variety of biological activities, and can cause cell autophagy and induce apoptosis effect characterized by a dose-dependent and time-dependent relationship [8]; in addition, it can clear free radicals of blood and superoxide radicals generated by xanthine and xanthine oxidase in aqueous solution [9]. In this regard, fullerenol has a higher biomedical value worth exploring. This study aimed to explore the possibility of producing safer and more effective anti-tumor effect with fullerenol combined with cisplatin by observing the changes of fullerenol, cisplatin and fullerenol combined with cisplatin on HeLa cells proliferation and comparing different fullerenol cisplatin and fullerenol cisplatin monotherapy effect, which provided experimental evidence and theoretical basis by using fullerenol in the treatment of cervical cancer

Objectives

Methods

Findings

Discussion

Conclusion