Abstract
Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.
Highlights
Autoimmune thyroid diseases (AITD) is a common organ specific Autoimmune diseases (AID), mainly including Graves' Disease (GD) and Hashimoto thyroiditis (HT), both of which have similar inheritance and immunity foundation, long time exposure to certain environmental factors may promote the population with specific gene background to suffer such a disease
3.1 Comparison of Th17 and Treg Cells &Its Cytokines with Transcription Factors Between the AITD Patients and the Control Group. This Research result discovers that theTh17 cell ratio, Th17/Treg ratio, IL-17, TGF-β and ROR-γt of AITD patients are higher than the NC group (P
The Th17 cell ratio, Th17/Treg ratio, IL-17 and ROR-γt of the GD patients are all higher than the NC group (P
Summary
Autoimmune thyroid diseases (AITD) is a common organ specific Autoimmune diseases (AID), mainly including Graves' Disease (GD) and Hashimoto thyroiditis (HT), both of which have similar inheritance and immunity foundation, long time exposure to certain environmental factors may promote the population with specific gene background to suffer such a disease. The change of Universal Salt iodization (USI) in the past 10 years and 10 years indicates that with the increase of iodine nutritious level, the occurrence rate of AITD rises . GD is a common endocrine disease and has the highest occurrence rate among all the hyperthyroidism types, accounting for about 85% of the hyperthyroidism [1]. The occurrence rate of GD in western countries is about 0.5%-2%, but for China, it is about 2%-3.0% [2]. The occurrence rate of HT in foreign countries is about 1%-2%, accounting for 22.5% of thyroid diseases. In China, the occurrence rate of HT is about 0.3%-10%, reaching as high as 22.5-40.7/100,000. Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD
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