RESEARCH ON OBTAINING LIQUID MYCELIA FROM PLEUROTUS SPP. STRAINS AND TESTING THEIR FRUITING POTENTIAL

Abstract

The paper focuses on the obtaining biotechnology and in vitro propagation of edible and/or medicinal macromycetes mycelia from the genus Pleurotus in submerged cultures in order to produce the biological “seeding” material - spawn. The quality and biological purity of the spawn is critical to the success of mushroom production and productivity. Starting from stock cultures from the collection of the RDIVFG Vidra, the mother cultures were obtained, the biological material being represented by two strains of Pleurotus ostreatus and one strain of Pleurotus citrinopileatus, each of them being cultivated in PDB + K2HPO4 (1,5g/l), pH 6.5 medium at different stirring speeds (0/100/150 rpm). The submerged growing biomass was used to inoculate wheat caryopsis, that evolved into spawn after complete colonization. The lignocellulosic substrate was obtained from wheat straws with the addition of nutritional supplements (wheat and maize bran and sunflower middlings) and distributed in the polypropylene bags. Their yielding capacity was verified in the fruiting chamber of the laboratory micro-mushroom farm test facility.