RESEARCH ON KRAS MUTATIONS IN PATIENTS WITH COLORECTAL POLYPS LARGER THAN 10 MM

Abstract

Objectives: Determine the prevalence of KRAS mutations using RNA samples and the association with endoscopic and histopathological images of colorectal polyps larger than 10mm. Subjects and methods: A cross-sectional study on 84 patients at the Gastroenterology-Hepatobiliary center - Bach Mai Hospital from 01/2017 - 12/2021. Perform a colonoscopy, select the largest polyp over 10mm in size to characterize and perform polypectomy, taking the specimen for histopathology according to WHO criteria 2010. Identification of KRAS gene mutations in tissue samples using RNA samples. Results: 10.7% of the KRAS gene is mutated. KRAS gene mutation rates tended to be higher in villous polyps compared to tubular polyps (33.3% vs. 9.2%) and high-grade dysplastic polyps compared to low-grade dysplastic polyps (23.1% vs. 8.6%), though no correlation between KRAS gene mutations and endoscopic imaging characteristics of polyps has been reported. Conclusion: KRAS gene mutations are not common in polyps larger than 10 mm but are related to the villious component and the degree of dysplasia on the histopathology of the polyp.