Relationship of K-ras and p53 gene mutation and superficial bladder cancer postoperative recurrence and survival rate

Abstract

Objective To explore the relationship of K-ras and p53 gene mutation and superficial bladder cancer postoperative recurrence and survival rate.Methods 137 bladder cancer patients subject to surgery were selected from December 2007 to June 2012.Twenty-nine normal tissue samples were selected as controls.Total DNA was extracted form bladder cancer tissues and normal tissues.p53 and K-ras gene mutations were analyzed by PCR-SSCP and gene sequencing.The relationship between p53 and K-ras gene mutation and superficial bladder cancer postoperative recurrence and survival rate was analyzed.Results p53 gene mutation was found in exons 7 and 8,and K-ras gene mutation in exons 1 and 2.p53 and K-ras gene single mutation rate in normal tissues was 3.45％ and 6.90％ respectively.The double mutation rate of p53 and K-ras gene in normal tissues was 0.The mutation rate of p53 and K-ras in bladder cancer tissues was 43.07％ (59/137) and 30.66％ (42/137) respectively.The double mutation rate of p53 and K-ras in bladder cancer tissues was 32.14％.The double mutation rate of p53 and K-ras in bladder cancer tissue was significantly higher than that in normal tissue (P ＜ 0.05).Postoperative recurrence in double mutation of p53 and K-ras was significantly higher than that in single mutation of p53 and K-ras (P ＜ 0.05).The 3-year survival rate in double mutation of p53 and K-ras was significantly higher than that in single mutation of p53 and K-ras (P ＜ 0.05).Conclusion p53 and K-ras gene mutation is one of the reasons leading to high postoperative recurrence and lower survival rate.p53 and K-ras gene mutation can be used as a clinical prognostic indicators. Key words: Bladder cancer; Postoperative recurrence ; p53 ; K-ras ; Gene mutation