Abstract
Objective To establish an HPLC fingerprint of Jinjing solid beverage, and provide experimental evidence of evaluating its quality. Methods Ten batches of Jinjing solid beverage were analyzed by HPLC under the gradient elution condition. A Kromasil C18 column (4.6 mm×250 mm, 5 μm) was used, and the flow phase was acetonitrile-H2O (acidified to 0.1% with phosphoric acid) with gradient elution, and the detection wavelength was 327 nm, and the flow rate was 1.0 ml/min, and the column temperature was 35 ℃. The data were evaluated by the similarity evaluation software for TCM fingerprint. Results The HPLC fingerprint of Jinjing solid beverage were established. Twelve common peaks including chlorogenic acid were identified with similarity of more than 0.9. Conclusion HPLC method is a reliable, available and quick method, that provides a means for controlling and evaluating the quality of Jinjing solid beverage. Key words: Chromatography, high pressure liquid; DNA Fingerprinting; Chlorogenic acid; Jinjing solid beverage
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