Abstract
Hibiscus Sabdariffa L. has important nutritional and medicinal value. Currently, a safe process extraction of reducing sugar from Hibiscus Sabdariffa L. calyx by biological complex enzyme system is seldom investigated. In this experiment, the pectinase and cellulase complex enzyme system was used to optimize the enzymatic hydrolysis of Hibiscus Sabdariffa L. and Box behnken was used to design the experiment. The results showed that there was significant difference in enzymolysis effect of Hibiscus Sabdariffa L under different conditions (p < 0.05). When the enzymolysis temperature was 45 °C, pH 4.5, and the mass volume ratio of material to liquid was 5g:100ml, the optimum conditions were obtained by response surface methodology: the addition amount of pectinase and cellulase complex enzyme was 0.011 g/kg, the ratio of mixed enzyme was 1.36:1, the enzymolysis time was 2.09 h, and the reducing sugar content of Hibiscus Sabdariffa L extract was 3.56 mg/ml. The experimental results of reducing sugar content of Hibiscus Sabdariffa L (3.54 ± 0.1mg / ml) were in good agreement with the predicted value of the equation fitted by response surface. The mixed enzyme system of pectinase and cellulase has the advantages of environmental protection and energy saving, which can provide guidance for industrial production.
Highlights
Hibiscus sabdariffa L., known as kaketti, luoshenhua, luoshenkui, shanqie, etc., is an annual shrub of Malvaceae wood mold genus, which is produced in Africa and has been planted for hundreds of years
The results showed that cellulase could obviously improve the light transmittance of the extract, which indicated that the extract of Hibiscus Sabdariffa contained a certain amount of fiber and hemifiber
The results showed that the extraction efficiency of pectinase mixed with cellulase was higher than that of single enzyme, and the extraction effect of pectinase was better than that of cellulase
Summary
Hibiscus sabdariffa L., known as kaketti, luoshenhua, luoshenkui, shanqie, etc., is an annual shrub of Malvaceae wood mold genus, which is produced in Africa and has been planted for hundreds of years. It is widely distributed in tropical and subtropical regions of the world and is a tropical and subtropical economic crop with a variety of economic uses. Hibiscus Sabdariffa is a ornamental plant, and has important nutritional health care and medicinal value. The unique nutritional value, healthcare and medicinal value of Hibiscus Sabdariffa determine that Hibiscus Sabdariffa has broad development and application prospects (Dhar et al, 2015). Some studies on the chemical constituents and pharmacological effects of Hibiscus Sabdariffa calyx show that Hibiscus Sabdariffa calyx has antioxidant, anti-tumor (Arenas et al, 2016), cardiovascular protection (Carvajal-Zarrabal et al, 2012), liver protection, antihypertensive (Dhar et al, 2015), diuretic and antilithiasis effect (Chen et al, 2020a)
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