Abstract
We studied fatty acid composition of the bacterial leaven of bifidobacteria and propionic acid bacteria that were grown together, and in a monoculture on the lactose medium with the addition of soybean serum in the amount of 3 %. The polyunsaturated fatty acids cannot be synthesized de novo in the organism of mammals. For this reason, searching for the new sources of polyunsaturated fatty acids is a relevant problem of biotechnology. Microorganisms appear to be a promising raw material. They are capable of synthesizing and accumulating in the cells up to 70 % lipids, including free fatty acids. In the course of the accumulation of biomass of the examined strains it was revealed that the propionic acid bacteria enter symbiotic relations with bifidobacteria. In the consortium, density of the population of both types of microorganisms was larger than that in a monoculture. We employed the dynamic method of gas chromatography in experimental studies using a flame-ionization detector. The results obtained allowed us to establish that the bacterial leaven В.longum-Ya3 and P.shermanii-PS4 differed from other samples by the high content of unsaturated fatty acids. In this leaven, the amount of linoleic acid was the largest and made up 23.99 % of the total sum of fatty acids. The data received confirm the expediency of using the probiotic bacteria for constructing the effective biologically active additives that contain essential fatty acids.
Highlights
In recent years, many scientists have paid considerable attention to the concept of recovery and rejuvenation of the human body with the help of including in the ration of sourmilk products that contain probiotic cultures [1].The action mechanism of probiotics is predetermined by both the high content of viable cells and the accumulation of their extracellular metabolites, which strengthen the probiotic effect
Most attention is paid to the microbial synthesis of polyunsaturated fatty acids (PUFA)
Fatty acids were introduced into the ration of user by means of biologically active additives (BAD) rich in PUFA of plant and animal origin
Summary
Many scientists have paid considerable attention to the concept of recovery and rejuvenation of the human body with the help of including in the ration of sourmilk products that contain probiotic cultures [1]. Most attention is paid to the microbial synthesis of polyunsaturated fatty acids (PUFA). They possess the anticarcinogenic, antigenogenic and antioxidant properties, they are the immunnomodulators, and control increase in the deposit fat [2]. Scientists have always paid considerable attention to the role of fatty acids in human nutrition. Fatty acids were introduced into the ration of user by means of BAD rich in PUFA of plant and animal origin. That is why examining the fatty-acid composition of probiotic cultures is a relevant task of biotechnology
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