Reproductive toxicity induced by lead exposure: Effects on gametogenesis and sex steroid signaling in teleost fish

Abstract

Lead (Pb) is an emerging contaminant widely distributed in aquatic environments, which has serious effects on human and animal health. In this study, we determined whether Pb exposure affects gametogenesis, sex steroids, estrogen (ERα and ERβ), and androgen (AR) receptors. Adult specimens of Astyanax bimaculatus were exposed in duplicate to 15, 50, and 100 μg/L of lead acetate, whereas the control group was not exposed. After 28 days of exposure, fish were euthanized and samples of the gonads, liver, and blood were collected for analysis. The results indicated a reduction in the gonadosomatic index as well as the diameters of the vitellogenic follicles and seminiferous tubules in the exposed groups. Morphometry of gametogenesis revealed inhibition of the secondary oocyte growth and a reduction in the number of spermatozoa in the 50 and 100 μg/L Pb-treated groups. In females, plasma 17β-estradiol (E2) increased following 15 and 50 μg/L Pb treatment, whereas males exhibited an increase in E2 and 11-ketotestosterone following treatment with 15 and 100 μg/L Pb, respectively. Vitellogenin was significantly reduced in females exposed to 100 μg/L Pb, but metallothionein levels were unchanged. ERα, ERβ, and AR were immunolocalized in the somatic and germ cells, with increased ovarian expression of ERα and Erβ in the 100 μg/L Pb-treated group, but no significant difference in AR among the groups. In males, only ERα increased in the 100 μg/L Pb-treated group. These results indicate that Pb exposure impairs gametogenesis, disrupts estrogen receptor signaling, and affects the expression of major reproductive biomarkers in A. bimaculatus.