Reproductive physiology of Indian pangolin (Manis crassicaudata) in Pothwar Plateau, Pakistan

Abstract

Abstract The Indian pangolin (Manis crassicaudata) is an “endangered” mammal species that is restricted to four range countries of Asia. The ecology and breeding behavior of Indian pangolins is poorly understood due to a lack of scientific data. Due to heavy poaching and illegal killing, the species population has declined drastically in the last decade. Considering the fact that Indian pangolin has low reproductive output, it needs special conservation measures and authentic information about its breeding ecology. We estimated concentrations of three reproductive hormones from scats using ELISA kits in male and female pangolins inhabiting the Pothwar Plateau, Pakistan, to confirm its breeding pattern in its range. Testosterone levels were found elevated from May (0.59 ng/ml) until October (1.28 ng/ml). Oestradiol levels were raised from May (0.59 pg/ml) until December (2.64 pg/ml). Progesterone concentrations were relatively low from January until March but increased from April (4.85 pg/ml) through December (14.0 pg/ml) with highest concentrations (20.13 pg/ml) in September and October. Mean hormone (testosterone, oestradiol and progesterone) concentrations were elevated in winter compared to summer (p < 0.001). The current study is the first one to quantify hormonal levels in this species, and our findings establish the breeding pattern of Indian pangolin.