Reprint of “Response to saltstress in seagrasses: PIP1; 1 aquaporin antibody localization in Posidonia oceanica leaves”

Abstract

Posidonia oceanica meadows are sensitive to disturbance often associated with highly human-impacted coasts. A recent anthropogenic impact is due to hypersaline water produced by desalination plants of seawater that severely affects growth rate, induces necrotic lesions and enhances meadow mortality. To define the early effects directly correlated to salt regime variation on leaf tissue, we focused on aquaporins, water channel proteins of intracellular and plasma membranes. PIP aquaporins localization was carried out in leaf cross sections, using an antibody raised against the evolutionarily conserved N-terminal amino acid sequence of Arabidopsis thaliana PIP1;1 (which showed 84% of identity with P. oceanica PIP1;1 aquaporin), both in leaves kept in natural seawater and in leaves subjected to hypersaline treatment. In natural seawater conditions, the antibody was localized mainly in epidermis and weakly in vascular bundles and mesophyll tissues of leaves. A widespread increase of fluorescence was observed in leaves subjected to hypersaline regime. Hence, our results showed histological evidence of PIP1;1 peptide increase following salt stress. Our data also suggest that aquaporins are involved in osmotic balance maintenance in seagrasses.