Replacement of Fish Meal by Defatted Yellow Mealworm (Tenebrio molitor) Larvae in Diet Improves Growth Performance and Disease Resistance in Red Seabream (Pargus major).

Chiemi Miura,Takashi Ohta,Atsushi Ido,Atsushi Hashizume,Takayuki Takahashi,Takeshi Miura

doi:10.3390/ani9030100

Abstract

Simple SummaryYellow mealworm is a potential novel protein source for sustainable food production, especially for aquaculture. In this study, the intake of a diet including defatted mealworm larvae was compared with a control diet containing fish meal from anchovy in a feeding trial with red seabream. As a result, the growth of red seabream fed the diet including defatted mealworm larvae with complete replacement of fish meal was higher than that of fish fed the control diet. Moreover, red seabreams fed diets including mealworm larvae gained disease resistance against pathogenic bacteria. The defatting process is thought to be important for insect-based diets, and the potential functional benefits for cultured fish from the diets, such as acquiring disease resistance, are remarkable.Yellow mealworm (Tenebrio molitor) larvae are a potential alternative animal protein source for sustainable aquaculture. However, reports on the successful complete substitution of fish meal with yellow mealworm larvae in an aquaculture diet have been limited. In this study, we conducted a feeding trial with red seabream (Pagrus major) being fed diets with partial or complete replacement of fish meal with yellow mealworm larvae defatted with a hexane–ethanol solution. Feed intake in red seabream increased in accordance with yellow mealworm larvae inclusion, and diets including 65% defatted mealworm larvae (complete replacement of fish meal) showed significant growth promotion. The addition of the oil fraction from mealworm larvae to diets resulted in growth reduction, despite meeting the nutritional requirements of red seabream. Moreover, the survival rate of red seabreams fed diets with partial replacement of fish meal with mealworm larvae was significantly higher in a challenge test with pathogenic Edwardsiella tarda bacteria. The present study demonstrated that yellow mealworm larvae are not merely an alternative animal protein, but have potential as functional feed ingredients for aquaculture production.

Highlights

Due to the rapid expansion of global aquaculture production, the development of alternative protein sources in feed for carnivorous fishes has become quite an urgent issue
Based on the evidence published to date, a diet with 50% to 70% replacement of fish meal (FM) with dried full-fat mealworm larvae resulted in similar growth to the FM diet in gilthead seabream (Sparus aurata) [7], European seabass (Dicentrarchus labrax) [8], black spot seabream (Pagellus bogaraveo) [9], and rainbow trout (Oncorhynchus mykiss) [10,11], and only 12% replacement for FM was achieved with defatted mealworm larvae in pearl gentian grouper (Epinephelus lanceolatus × Epinephelus fuscoguttatus) [12]
The crude protein and crude lipid levels in DMW-1 and defatted mealworm larvae produced in Shangdong (DMW-2) were similar to those of FM owing to the defatting process

Summary

Introduction

Due to the rapid expansion of global aquaculture production, the development of alternative protein sources in feed for carnivorous fishes has become quite an urgent issue. Some studies have reported the potential as an alternative feed ingredient of yellow mealworm larvae (MW; Tenebrio molitor Latreille) (Tenebrionidae: Coleoptera) in aquaculture production [1,2,3,4]. This insect appears to possess adequate amino acid profiles for use in fish feed [1,2,3,5,6], reports on the successful complete substitution of fish meal (FM) with MW have been limited. The MW’s potential in diets for red seabream (Pagrus major Temminck and Schlege) (Perciformes: Sparidae) has not been investigated

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