Abstract
E. coli ATCC 11229 was exposed to Cl under demand-free conditions, and surviving colonies examined. Survivors at low degrees of inactivation did not appear to possess altered sensitivity, even when successively exposed and subcultured through 8 passages. Survivors at high degrees of inactivation appeared to have a greatly increased sensitivity towards free Cl. The type culture, and the sensitive strain had identical growth curves, microscopic appearance, and biochemical traits. The initial rate of inactivation at pH 7 appeared to follow saturation-type kinetics, previously reported for organisms and Cl. Implications for water treatment and reuse are discussed, as well as possible mechanisms explaining the findings of this study.
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