Identification of cysteine and lysine residues present at the active site of beef liver glutamate dehydrogenase by o-phthalaldehyde

Abstract

Beef liver glutamate dehydrogenase (GDH) is inactivated by the bifunctional reagent, o-phthalaldehyde. The initial rate of inactivation follows pseudo first-order kinetics. The reaction of the enzyme with o-phthalaldehyde results in isoindole derivative formation which is characterized by typical fluorescence emission and excitation maximum at 410 nm and 337 nm, respectively. The inactivation of GDH by o-phthalaldehyde is partially prevented by α-ketoglutaric acid, whereas NADH does not provide any protection. This clearly indicates that cysteine and lysine residues are located near the α-ketoglutaric acid binding center. The dissociation constant of 2.2 mM was obtained for enzyme-α-ketoglutaric acid complex. Stoichiometry of o-phthalaldehyde binding with glutamate dehydrogenase showed that the formation of approximately one isoindole derivative per subunit of glutamate dehydrogenase is accompanied by complete loss of activity.