Abstract
Objective To observe the effect of co-transplantation of human umbilical cord mes-enchymal stem cells (hUCMSCs) and autnlognusly activated Schwann cells (AASCs) on the recovery of acute spinal cord injury (SCI),as well as the influence of AASCs on the survival and differentiation of hUCMSCs. Methods hUCMSCs were isolated from human umbilical cord,cultured and purified in vitro. Unilateral saphenous nerves were ligated to isolate AASCs. Eighty female Wistar rats [(77±2) d] were randomly as-signed to 4 even study groups: DMEM control,hUCMSCs transplantation,AASCs transplantation;and co-transplantation. The acute spinal cord contusion models (T10) were established by IMPACTOR MODEL-Ⅱ device. Recovery of the lower extremity was observed using Basso- Beattie- Bresnaban (BBB) locomotor scoring system,and immunohistnchemical stain to detect the survival and differentiation of hUCMSCs. Re-generation of the corticospinal tract (CST) was observed using the biotinylated dextran amine (BDA) tracing. Results The BBB scores in cell transplantation groups were significantly higher than in the DMEM control group 4 weeks after injury (P<0.05 ) . The co-transplantation group had a higher score than the hUCMSCs group(P<0.05) . Both the number of surviving hUCMSCs and the positive response area in im-munohistochemistry staining were significantly larger in the co-transplantation group than in other groups 6 weeks after injury(P<0.05) . The BDA anterograde tracing showed more regenerating nerve fibers got through the injured area in the co-transplantation group which also had the smallest injured cavity,compared with other groups. Conclusions AASCs can promote survival of hUCMSCs and their differentiation into neural cells. Co-transplantation of hUCMSCs and AASCs may be more effective in promoting functional re-covery and axonal regeneration after SCI. Key words: Spinal cord injury; Cell transplantation; Neural regeneration; Umbilical cord mesenchymal stem cells; Schwann cells
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