Abstract
BackgroundAlveolar cleft is a type of cleft lip and palate that seriously affects the physical and mental health of patients. In this study, a model of the alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (HUC-MSCs) on the repair of alveolar cleft bone defects.MethodsA model of alveolar clefts in rabbits was established by removing the incisors on the left side of the upper jaw bone collagen particles combined with HUC-MSCs that were then implanted in the defect area. Blood biochemical analysis was performed 3 months after surgery. Skull tissues were harvested for gross observation, and micro-focus computerised tomography (micro-CT) analysis. Tissues were harvested for histological and immunohistochemical staining. The experiments were repeated 6 months after surgery.ResultsBone collagen particles and HUC-MSCs showed good biocompatibility. Bone collagen particles combined with HUC-MSCs were markedly better at inducing bone repair and regeneration than bone collagen particles alone.ConclusionsCombining HUC-MSCs with bone collagen particles provides a simple, rapid and suitable method to fill a bone defect site and treat of alveolar cleft bone defects.
Highlights
Alveolar cleft is a type of cleft lip and palate that seriously affects the physical and mental health of patients
The purpose of this study is to investigate the feasibility and effectiveness of bone collagen particles inoculated with HUC-MSCs in a rabbit model of alveolar clefts
The results indicate that HUC-MSCs combined with bone collagen particles may be a reliable alternative therapy for the repair of alveolar bone defects
Summary
Alveolar cleft is a type of cleft lip and palate that seriously affects the physical and mental health of patients. Alveolar cleft is a common disease that affects the normal eruption of teeth and the development of the jaws, and affects the physical and mental health of patients [1]. It is very important to establish a repeatable animal model of alveolar cleft that is similar to human alveolar clefts disease. This method can provide a theoretical basis for the occurrence and development of the disease, as well as provide a good scientific research foundation for the repair of alveolar clefts. Studies in our laboratory have proved that rabbits can be used to establish animal models of the alveolar cleft [9]. Rabbits were selected as experimental animals to establish an animal model of alveolar clefts in the present study
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