Repair capacity of perennial ryegrass (Lolium perenne L.) based on arbuscular mycorrhizal fungi on the in uranium contaminated soil

Abstract

This study is to research effects of arbuscular mycorrhizal fungi (AMF) on the repair capacity of perennial ryegrass (Lolium perenne L.) in the uranium-containing soils. Perennial ryegrass plants were harvested 60 days after sowing in soils. Photosynthetic pigments, soluble proteins, and malondialdehyde were determined by ethanol extraction method, Coomassie brilliant blue method, and thiobarbituric acid method, respectively. Activities of alkaline phosphatase and succinate dehydrogenase were also determined. And, cellular and subcellular distributions of uranium in plant epidermal cells were observed with Hitachi H-7650 transmission electron microscope. AMF influents photosynthetic pigment levels of perennial ryegrass. AMF increases the soluble protein levels in perennial ryegrass and represses the uranium-induced increase of malondialdehyde levels in perennial ryegrass. It suggested that AMF can effectively increase the ability of perennial ryegrass on repairing the uranium-related soil contamination.