Effects of arbuscular mycorrhizal fungi on the repair capacity of perennial ryegrass (Lolium perenne L.) in the uranium-containing soils

Abstract

This research inclines to study the ramification on arbuscular mycorrhizal fungi (AMF), including Glomusclaroideum (G. claroideum) and G. mosseae strains as well as the repair process of perennial ryegrass (Lolium perenne L.) in the uranium-containing soils. Three times at 120°C for an hour was required to disinfect the soil mixture. Spraying the uranium (U3O8) into the soil mixture and mixing until the ingredients were thoroughly blended, serving the purpose of obtaining the uranium-containing soil mixture with 5 mg/kg U3O8. A week later, AMF was inoculated into every flowerpot and chamber facility. Seeds of perennial ryegrass were cultivated with thirty seeds in each flowerpot or each indoor equipment in a fortnight’s time. Perennial ryegrass plants tended to be harvested after sowing and meeting the conditions of 60 days. Three substances are detected by three different detection means(Photosynthetic pigments, soluble proteins, and malondialdehyde correspond to ethanol extraction method, Coomassie brilliant blue method, and thiobarbituric acid method, respectively). Additionally, testing included activities of alkaline phosphatase and succinate dehydrogenase as well. Moreover, Hitachi H-7650 transmission electron microscope performed the function of observing cellular and subcellular distributions of uranium in plant epidermal cells. Photosynthetic pigment standards of perennial ryegrass are subject to AMF, which not only has a tendency to enhance the soluble protein levels in perennial ryegrass, but also gains the possibility of holding up the uranium-induced improvement of malondialdehyde levels in perennial ryegrass. G. mosseaes is properly more efficacious than G. claroideum in the attenuation of uranium damages in cell structures of perennial ryegrass. Our results demonstrate that AMF is capable of developing the capacity of perennial ryegrass on repairing the uranium-related soil contamination.