Reno-protective Effects of Brusatol Against Renal Ischemia Reperfusion Injury

Abstract

Purpose: The aim of this study was to determine the potential beneficial effects of brusatol treatment on oxidative kidney injury induced by bilateral renal ischemia reperfusion (RIR) method.
 Material and Method: In the existing study, experimental animals were randomly assigned to 4 groups as sham, renal ischemia reperfusion (RIR), DMSO and brusatol groups. Sham group; the back region was opened by incision and then sutured but no ischemia reperfusion (IR) model was established. In RIR group, 1 hour of ischemia following 24 hours of reperfusion was formed. In DMSO group, 0,3 ml, 1% DMSO was administered intraperitoneally for each rat once in two 2 days for 10 days and a last dose was applied 30 minutes before reperfusion. Then IR model was carried out as told in RIR group. In brusatol group, brusatol was applied intraperitoneally as 0,5 mg/ml for each rat every second days for 10 days before the experiment. The last dose was administered 30 minutes before reperfusion and IR was fulfilled as depicted in RIR group. Following reperfusion period, rats were immolated and renal tissues were isolated.
 Results: TNF-α, MDA and IL-1β levels, OSI, TOS and MPO values were significantly raised but TAS and SOD levels were declined in RIR and DMSO groups compared to sham group. On the other side, TAS and SOD increased while OSI and TOS values, activity of MPO and TNF-α, MDA and IL-1β levels were significantly reduced in brusatol+I/R group due to brusatol therapy compared to sham and DMSO groups. 
 Conclusion: Consequently, brusatol demonstrated protective effects against RIR induced oxidative kidney injury in rats.