RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM GENES POLYMORPHISM AND RELATION WITH VASOPRESSORS IN ESSENTIAL HYPERTENSION AMONG DAGESTAN INHABITANTS

Abstract

Aim. Investigation on the prevalence of genotypes polymorphisms of reninangiotensin- aldosterone system and beta2-adrenoreceptors in essential hypertension (EH) of I, II, III grades inDagestan population, and evaluation of the relation of genotype expression with vasopressors levels in blood. Material and methods. Totally, 98 patients included, with the diagnosis “essential hypertension of I, II, III grades”. The genotypes were studied, of polymorphisms Тhr174Мet and Мet235Тhr gene AGT , polymorphism A1166C gene AGTR1 and polymorphisms Arg16Gly and Gln27Glu gene ADRB2 . Test of the mentioned polymorphisms was done with the allele-specific polymerase chain reaction, making to assess the absence or presence of a mutation allele in hetero- and homozygous form. The level of angiotensin (AT) II, endothelin (ET) 1-21 and aldosterone (AS) in serum was measured with the hard-phase immune-enzyme assay. The level of angiotensin-converting enzyme (ACE) was measured with enzymatic method. Statistics was done with the software Statistica v. 6.0 and “Biostat4.03”. Results. EH of I, II, III grades in Dagestan population was associated with significant increase of the prevalences (criteria χ2) of genotypes АА and СС of polymorphism A1166C gene AGTR1 , genotype Arg/Gly of polymorphism Arg16Gly gene ADRB2 , genotype Gln/Gln of polymorphism Gln27Glu gene ADRB2 and genotype ММ of polymorphism Thr174Met gene AGT . In the carriers of the genotypes mentioned, relative risk of EH onset was significantly higher than in controls. The level of vasopressors ATII, ET 1-21, AS and ACE showed a controversial dynamics in Dagestan population. Serum levels of ET 1-21 and ACE were significantly higher or had tendency to increase, but AT II and AS decreased in both cases, comparing to the controls (р<0,05, р<0,01). Most number of significant direct correlations was found in polymorphisms of the genes AGT , AGTR1 and ADRB2 and level of serum ET 1-21. Conclusion. Significant increase of the prevalence of the studied genotypes polymorphisms of genes AGT, AGTR1 and ADRB2 in EH of I, II III grades represents a definite specifics of mutational process involving the mentioned genes inDagestan population. These processes are associated with significant changes in vasopressors levels in serum, that is a witness for pathogenetic relationship of the mentioned polymorphisms and pressoric contour of EH inDagestan population.