Abstract
The accumulation lipid from oleaginous microorganisms is recognized as a second generation fuel. Biooil is known to as intracellular product of oily yeast utilizing various carbon substrates and converting different quantities of lipids in the form of triacylglycerols. This second generation fuel can be used to make biodiesel via a transesterification process. This study investigated the morphological characteristics of eight strains of Thai oleaginous yeasts via microscopy and analyzed the fatty acid profiling of yeasts cultured in three carbon sources: glucose, sugar cane molasses and crude glycerol in order to estimate biodiesel properties. To approach this goal, batch fermentations were used to culture eight yeast strains,Rhodosporidium toruloidesTISTR 5123, TISTR 5154, TISTR 5149,Yarrowia lipolyticaTISTR 5054, TISTR 5151, TISTR 5621,Rhodotorula glutinisTISTR 5159 andRhodotorula graminisTISTR 5124 for 96 h under 30°C at 250 rpm. Result revealed that eight yeast strains contained significant amounts of fatty acids and lipids and accumulated mainly palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C 18:1) and linoleic acid (C18:2), and they are suitable for the production of biodiesel. Fatty acid productions and profiles indicated that these yeast strains can be potentially used as the triacylglycerols producers for biodiesel production.
Highlights
Soybean, rapeseed and palm oil are the main feedstock for biodiesel production in America, Europe and Asia [1]
The similarity of triacylglycerols (TAGs) of the biooil from vegetable oils have increasing the interest to use these fatty acid compositions to further synthesis a mixture of fatty acid methyl esters (FAMEs) in to biodiesel [3]
Rhodosporidium toruloides TISTR 5123, 5154 and 5149 Yarrowia lipolytica TISTR 5054, 5151, 5621, Rhodoturola glutinis TISTR 5159 and Rhodoturola graminis TISTR 5124 were purchased from Thailand Institute of Scientific and Technological Research
Summary
Soybean, rapeseed and palm oil are the main feedstock for biodiesel production in America, Europe and Asia [1]. These feedstocks are commonly applied to biodiesel fuel production via transesterification. Oleaginous microorganisms are including bacteria, yeast, fungi and algae. For oleaginous yeast which is the well-known microbial oil organism because they produced more than 20% of their biomass into intracellular lipid. These microorganisms produce triacylglycerol and many type of fatty acids depending on cultivation media and stress under the growth.
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