Abstract
AS INCREASING numbers of expanded criteria and non–heart-beating donor kidneys are procured, the need for a reliable, accurate test of viability prior to transplantation becomes more acute. An in vitro model of renal cellular ischaemia would facilitate the study and assessment of viability tests and renoprotective compounds. An experimental system for anoxic culture and manipulation of cultured cells has been established and used to study renal cell viability after an ischaemic insult.
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