Removal of textile dye Reactive Black 5 from aqueous solution by adsorption on laccase-modified silica fume

Abstract

AbstractTextile wastewater with the dye is released in various ways into the environment and it causes significant environmental problems. Due to the stability of modern dyes, conventional biological treatment methods for industrial wastewater are ineffective, resulting in an intensively colored discharge from the treatment facilities. Thus, there is a need for developing more effective treatment methods to eliminate dyes from the waste stream at its source. The most effective methods of dye removal from wastewater are adsorption and the use of enzymes. In this study, the removal of Reactive Black 5 (RB5) from aqueous solutions has been studied using silica fume (SF) waste material after its modification with laccase from Russulaceae (Lactarius volemus). Laccase was purified by using saturated precipitate (NH4)2SO4, DEAE-cellulose and immobilized on SF. Batch adsorption experiments have been performed as a function of pH, contact time, temperature, and adsorbent dosage. The optimum results were obtained a...