Removal of Pyrogens by lon Exchange, Adsorption and Membrane Permeation

Abstract

Pyrogenic aqueous solutions were prepared from that containing 5fe250 partly caramelizing glucose or from the deionized water containing O.33 or 3.3ppm of bacto-lipopolysaccharide SL abortus equi. These aqueous solutions, which were found to be highly pyrogenic, were passed through ion exchange reg. ins, adsgrbents and hyperfiltration membranes to test the degtee of removal of pyrogens. Some of the macroreticular ion exchange resins and gelular anion exchange resins were effective tg some ex. tent for the removal of pyrogens (Table 3 and 5). Neither activated carbon nor synthetic adsorbents proved to be effective for the remeval 6f pyrogens. With regard to membrane permeation, the membranes used in this work were found to be very effectiye-for. the removgl o-f pyrogens (Table 4, .6 and 8). The reproducibility of these membranes used for the removal of pyregens was good.