Removal of phenolics in olive mill wastewaters using the white-rot fungus Pleurotus ostreatus

Abstract

Olive mill wastewaters (OMW) present a major environmental problem. The large amounts generated, combined with the high phenols and chemical oxygen demand concentrations, are the main difficulties in finding a solution for the management of these wastewaters, which are dangerous for the environment. The phenols, which are contained in the OMW have a structure similar to lignin, which makes them difficult to biodegrade. Lignin can be degraded only by a few microorganisms, such as “white-rot” basidiomycete, which produce manganese (MnPs) and lignin peroxidases (LiPs) and laccases that are responsible for the oxidisation of lignin compounds. The capability of Pleurotus ostreatus to degrade phenols of OMW in different conditions such as in sterilized and thermally processed (at 100°C) wastewater, with and without dilution, is investigated in this work. According to the experimental results P. ostreatus removed phenols from the culture medium, under all different conditions that were examined. The degradation of phenols reached up to 78.3% for the sterilized and 50% diluted OMW, 66.7% and 64.7% for the thermally processed OMW, with and without dilution, respectively. The effect of pre-treatment of OMW on the performance of anaerobic digestion is also assessed, as methanogenic bacteria are seriously affected by the presence of phenol compounds. The pre-treated wastewater was shown to be more amenable to a subsequent anaerobic digestion.