Removal of cholesteryl esters from diet-induced atherosclerotic lesions in two long-term studies in Rhesus monkeys

Abstract

In two long-term studies of dietary-cholesterol-induced atherosclerosis lesions in rhesus monkeys, we determined the fatty acid composition of cholesteryl esters in the arterial intima-media preparations. In the first study (2-year study) monkeys were fed an atherogenic diet for 2 years; in the second study, (5-year study), monkeys were fed the atherogenic diet for about 5.4 years. Dietary cholesterol was removed from regressed animals and the regression periods in the 2-year study were 30 and 52 weeks and in the 5-year study were 20, 30, 52, 101, and 191 weeks. In both studies in thoracic and abdominal aortic segments, the percent removal of 18:2 cholesteryl ester (CE) was small, whereas percent removal of 18:1 CE and other CEs was much higher. We postulate that 18:2 CE is not hydrolyzed to the extent comparable to 18:1 and other CEs in lesions. Perhaps 18:2 CE is not a preferred substrate for arterial neutral cholesterol ester hydrolase.