Abstract
Background Papanicolaou stained (Pap) smears in fine needle aspiration cytology play an important role for studying chromatin details of the cells and also ensures its optimal resemblance with the cell nuclei on histological sections. The conventional Pap Stained smears are ethanol fixed before drying. But delay in fixation due to any reason results in drying artifacts in the stained smears making it an inept smear for further examination. Aims & objectives: To study the role of rehydration fluid for removal of air drying artifact. Material and methods: Fifty fine needle aspiration smears from different organs with drying artifact in Pap Stained smears were included in the study. The smears were destained, rehydrated with rehydrating fluid for two hours and were restained with Pap stain after fixation with 95 % alcohol. Results : Smears treated with this solution showed reversal of the air drying artifact with better nuclear details and background material staining. Conclusion: Rehydration of destained Pap smears with rehydrating fluid followed by restaining results in optimal staining of the nuclear and background details preventing the material loss and making the smears appropriate for staining.
Highlights
Fine needle aspiration cytology (FNAC) is commonly performed for early and inexpensive diagnosis of various tumors and tumor like conditions
The aim of the present study is to study the role of rehydration fluid in removal of air drying artifacts in staining
A total of fifty cases comprising of FNAC from thyroid, lymph nodes, soft tissue, skin adnexal lesions and breast were included in the study
Summary
Fine needle aspiration cytology (FNAC) is commonly performed for early and inexpensive diagnosis of various tumors and tumor like conditions. Depending on institutional preferences wet fixed papanicolauo stain or H&E and/or air dried Romanowsky’s stains are routinely are done. Both the pap stain and Romanowsky’s stain are complementary to each other. Material and Methods: Fifty fine needle aspiration smears from different organs with drying artifact in Pap Stained smears were included in the study. Results: Smears treated with this solution showed reversal of the air drying artifact with better nuclear details and background material staining. Conclusion: Rehydration of destained Pap smears with rehydrating fluid followed by restaining results in optimal staining of the nuclear and background details preventing the material loss and making the smears appropriate for staining
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
