Abstract
Background: After the first evolution of Papanicolaou smear, it has been successfully used to screen cervical cancer. With modifications in the staining method of Pap stain, the lesser staining time and unequivocal cell morphology has been achieved by Ultrafast Papanicolaou stain. The aim of this study is to compare the UFP stain and SP stain in cervical pap smears on the basis of background, cell morphology, nuclear details and overall staining.Materials and methods: This is a prospective hospital based study conducted in a tertiary hospital, Nepal Medical College over a period of six months (January 2017 to June 2017). Cervical pap smears received in the department of Pathology is subjected to Standard papanicolaou (SP) stain and Ultrafast papanicolaou (UFP) stain for comparison.Results: Total of 368 cervical pap smears were subjected to SP stain and UFP stain. Mean age of the patient was 36.57+-10.06 years. The quality of UFP stain was assessed by considering the background, cell morphology, nuclear details and overall staining. Difference in regards to the interpretation and background was not significant when SP stain was compared to UFP stain. However, cell morphology, nuclear details and overall staining were far better in smears stained with UFP stain.Conclusion: Pap smear is the simple and effective screening tool for cervical lesions. With modifications in staining method and introduction of UFP stain, the result is lesser staining time and excellent morphological quality. Hence, UFP stain has become the choice of stain and can be used on a regular basis.
Highlights
Cervical Pap smear is a simple, convenient, cost effective and reliable test for easy screening of cervical lesions
The time taken for staining the smears with Ultrafast Papanicolaou (UFP) stain and Standard Papanicolaou (SP) stain was 2.5 minutes and 20 minutes respectively
The quality of these two stains was assessed based on the background, preservation of cell morphology, nuclear details and overall staining
Summary
Cervical Pap smear is a simple, convenient, cost effective and reliable test for easy screening of cervical lesions. There has been a dramatic reduction in the incidence and mortality of invasive cervical cancer world wide.[1,2,3] Pap stain is the preferred stain for gynaecological and non gynaecological cytology smear.[4,5] Pap stain yields polychromatic transparent staining reaction with crisp nuclear and cytoplasmic features. It was first developed by Dr George Nicholas Papanicolaou in 1942 to know the variation in cellular maturity and metabolic activity in vaginal smears.[4] Pap stain clearly distinguish between. Data were entered in Microsoft office excel 2003 analyzed using Statistical Package for the Social Sciences- 16 (SPSS 16)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
