Remineralized Evaluation of Nano- Hydroxyapatite to Artificial Caries

Abstract

The aim of this study is to describe the remineralization effect of the nano-HA on artificial caries. The artificial dental caries are made by using sour solution, and constructing an artificial mouth' plaque culture system in vitro respectively. The scanning electron microscope (SEM) and the optical microscope are used to observe the surface of enamel specimens. The effect of remineralization is assessed with the polarized light microscopy (PLM) and the confocal laser scanning microscope (CLSM) by measuring three lesion parameters (area, total and average fluorescence). The SEM analysis shows that the cavities and defects of enamel surface are decreased and many mineral salts are sedimentated, which indicate that the nano-HA could promote remineralization for the demineralized enamel. The inhibitory effect of the spherical nano-HA on dental caries is detected in the artificial mouth test. The CLSM shows that demineralization of enamel is reduced by adding the spherical nano-HA in artificial mouth. The results show that the spherical nano HA have a remineralization effect on the artificial dental caries dramatically, and can prevent and decrease caries.