Remineralization of enamel sub-surface lesion using casein phosphopeptide amorphous calcium phosphate (CPP-ACP) - a quantitative energy dispersive X-ray analysis (EDAX)

Abstract

This study aimed at quantitatively evaluating the remineralization potential of casein phosphopeptide -amorphous calcium phosphate (CPP-ACP) paste on artificial enamel sub-surface lesion using Energy Dispersive X-rayAnalysis (EDAX).<br>60 enamel specimens were prepared from extracted human molars. All specimens were evaluated for mineral (calcium and phosphorous) content (wt %) using EDAX. The specimens were placed in demineralizing solution for 48 hrs to produce artificial carious lesions. Mineral content was again measured using EDAX. The specimens were then randomly assigned to 3 study and 1 control group (incubated in artificial saliva after demineralization), in which each group except the control was treated with remineralizing paste (10 % CPP-ACP paste) for 1. 5 and 10 days twice daily for 3 minutes, followed by incubation in artificial saliva at 370°C The control group received no treatment with remineralizing paste. After the remineralization treatment mineral content (wt %) of samples was measured using EDAX.<br>The study groups showed an increase in the mineral content as compared to demineralized samples. No change was seen in the control group. 10 % CPP­ACP paste could significantly remineralize the artificial enamel sub-surface lesion in vitro and the remineralization potential was dose dependent with.