Abstract
Abstract Previous studies have suggested that resistin-like molecule beta (RELM-beta) contributes to protective immunity against intestinal nematode parasites by directly interfering with adult worm feeding in the lumen. Other studies have suggested that RELM-beta may also function in the mucosal milieu by regulating Th1/Th2 cytokine production. In our studies, we examined the development of the memory Th2-type response to the murine intestinal nematode parasite, Heligmosomoides polygyrus (Hp). Mice were inoculated with Hp, drug-cleared at day 14, and several weeks later given an Hp secondary inoculation. Consistent with previous studies, adult worm burden was increased in Hp inoculated RELM-beta KO compared to inoculated WT controls. Analysis of cytokine gene (RT-PCR) and protein (ELISPOT) expression in draining mesenteric lymph nodes (MLNs) showed reductions in Th2 cytokine levels in RELM-beta KO compared to WT mice at day 7 after Hp secondary inoculation. At this time point spleen weight and total numbers of MLN CD4+ T cells and B cells were also reduced in RELM-beta KO compared to WT mice. Analysis of sorted CD4+ T cells showed reduced IL-4 expression in RELM-beta KO mice compared to WT mice at day 7 after secondary inoculation. These studies suggest that RELM-beta may affect protective immunity through multiple mechanisms, including enhancement of theTh2 cytokine response.
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