Abstract
The validity of the effective linear calibration (ELC) method in gel permeation chromatography of polymers has been checked by means of computer generated chromatograms. The ratio Mw/Mn calculated using ELC form an uncorrected chromatogram is always smaller than that determined by means of the true calibration dependence, but the extent of this correction for longitudinal spreading depends on the polydispersity of both the original calibration standard and the analyzed polymer, and also on the separation efficiency of the given column. If the respective molecular weight averages of the analyzed sample and of the calibration standard differ considerably, the effective linear calibration method leads to serious systematic errors in the calculated average molar mass values.
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