Release of microparticles from native coronary arteries and saphenous vein grafts in patients with elective stent implantation under protection with an aspiration device

Abstract

ObjectiveStent implantation into coronary vessels impacts on downstream microvascular function and induces the release of particulate debris and soluble substances, which differs between native right coronary arteries (RCAs) and saphenous vein grafts on right coronary arteries (SVGRCAs). We have now quantified the release of microparticles (MPs) during stent implantation into RCAs and SVG‐RCAs.MethodsPatients with a stenosis in their RCA or SVG‐RCA, respectively (n = 14/14), were enrolled. Coronary aspirate was retrieved during stent implantation with a distal occlusion/aspiration device. Platelet‐derived MPs (PMPs) were distinguished by flow cytometry as CD41+, endothelium‐derived MPs (EMPs) as CD144+, CD62E+ and CD31+/CD41‐, leukocyte‐derived MPs as CD45+, and erythrocyte‐derived MPs as CD235+.ResultsIntracoronary PMPs and EMPs were increased after stent implantation into their RCAs and SVG‐RCAs (CD41+: 2729.6±645.6 vs. 4208.7±679.4 and 2355.9±503.9 vs. 3285.8±733.2 nr/μL; CD144+: 451.5±87.9 vs. 861.7±147.0 and 444.6±74.8 vs. 726.5±136.4 nr/μL; CD62E+: 1404.1±247.7 vs. 1844.3±378.6 and 1084.6±211.0 vs. 1783.8±384.3 nr/μL, P<0.05), but not different between RCAs and SVG‐RCAs.ConclusionStenting in RCAs and SVG‐RCAs is associated with a substantial release not only of PMPs, but also of EMPs.