Abstract
Publisher Summary This chapter explores the release of complex carbohydrates into culture medium by cultural hamster cells. In a study described in the chapter, normal, secondary fibroblasts of hamster embryo (HE 1) were labeled with [3H]glucosamine for either 24 or 48 h, washed extensively with unlabeled medium, and allowed to release labeled compounds into the culture medium for 48 h, while subconfluent and growing. Sepharose 6B gel-filtration of the labeled products in the medium revealed three zones of eluate, namely exclusion volume peak, intermediate mol. wt. peaks, and low mol. wt. peak. Comparison—qualitatively and quantitatively—of the uptake of [3H] glucosamine and the release of labeled compounds by normal cells and several transformed lines shows remarkable similarity, with significant differences observed only in the ion-exchange behavior of Zone 3 labeled material.
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