Abstract
A new, stable and accurate high performance liquid chromatography ultraviolet spectrophotometric method for quantitation of Ciprofloxacin in human plasma was developed and validated. The chromatographic separation was performed on a reversed phase BDS (base deactivated silica) C18 analytical column; 150 × 4.6; 5 μm particle size. The mobile phase consisted of a mixture of 1 ml triethylamine (TEA)/1L, water and acetonitrile (80:20 v/v). The pH of mobile phase was adjusted to 2.5 by using phosphoric acid and the detector was set at a wavelength of 280 nm. Accuracy, precision, linearity and stability of the analytical method were done. The regression equations are linear (R 2 was 0.999) over the calibration curve concentration range of (0.10-8.0) µg/ml. Inter and Intra precision (% CV) were less than 6.102% and 6.82% respectively.
Highlights
The mobile phase consisted of a mixture of 1 ml triethylamine (TEA)/1L, water and acetonitrile (80:20 v/v)
Two different tablet doses of Ciprofloxacin (750 and 1000) mg were administrated to 28 healthy male volunteers in cross over design, blood samples were taken from the participant at different time intervals and Ciprofloxacin concentrations were analyzed
Ciprofloxacin which regard as antibacterial agent [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3 quinolinecarboxylic acid] is a fluoroquinolone derivative (Figure 1) related structurally to nalidixic acid
Summary
Ciprofloxacin which regard as antibacterial agent [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3 quinolinecarboxylic acid] is a fluoroquinolone derivative (Figure 1) related structurally to nalidixic acid. Precision, linearity and stability of the analytical method were done. Two different tablet doses of Ciprofloxacin (750 and 1000) mg were administrated to 28 healthy male volunteers in cross over design, blood samples were taken from the participant at different time intervals and Ciprofloxacin concentrations were analyzed. Absorption after oral administration is rapid; peak serum ciprofloxacin concentrations (Cmax) between 0.8 and 3.9 μg/ml are reached after 1 to 2 hours following oral administration of single dose of 250-750 mg.
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