Abstract
The use of an inhibitor (0.4 N (NH 4) 2SO 4) and an activator (sodium polydextran sulfate) of initiation of transcription in nuclei of frog embryos in vitro indicated that initiation sites for transcription are relatively A—T-rich. Comparison of incorporation of labeled ATP, UTP, and GTP, when initiation of transcription is prevented, indicates that initiation sites become relatively less A—T-rich as development proceeds. Inhibition of initiation of transcription in isolated nuclei blocks the normal increase in rate of transcription that occurs when cells enter the S phase, showing that an increased level of transcription depends upon a greater number of initiation sites.
Published Version
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