Abstract
Assessment of erythrocyte morphology is an important aid in diagnostic haematology. Anisocytosis which is changes in RBC size, correspond to changes in diameter and surface area (SA) of the cell, and are not always reflected in the Mean Corpuscular volume (MCV). This paper discusses the relationship of MCV with surface area and diameters (vertical and horizontal) in 2D views of erythrocytes of clinically healthy dogs using confocal microscopy. This information would be valuable for early detection of cellular changes in dogs in subclinical or clinical diseases. The average diameters, average SA, and MCV values of RBCs studied were in normal distribution and the mean values of 7.169 ± 0.648 μm for horizontal diameter (D1), 7.1245 ± 0.6646 μm for vertical diameter (D2), and 41.061 μm2 ± 6.866 for SA did not reveal a significant correlation or a strength of association with the MCV values, indicating that the MCV value has limitations as an objective measurement of detecting anisocytosis.
Highlights
Examination of blood is virtually the universal first step in the evaluation of health and disease in human and animal patients as physiological and pathological changes in most tissues are reflected directly or indire ctly in blood
The morphology of red cells is categorized according to colour, size, shape, intra or epicellular structures, and their arrangement on blood films, which are unique for each animal species (Ford, 2013;Thrall, 2006)
This paper compares the relationship of Mean Corpuscular Volume (MCV) with surface area (SA) of erythrocytes and diameters in 2D views of RBCs
Summary
Examination of blood is virtually the universal first step in the evaluation of health and disease in human and animal patients as physiological and pathological changes in most tissues are reflected directly or indire ctly in blood. Changes in RBC size correspond to changes in cell diameter and not necessarily to changes in RBC volume, and changes in cell volumes are not always reflected in the MCV. Changes in diameter may not be reflected in the MCV obtained as a direct measurement from automated hematology analyzers, which is the average volume of RBCs in a mixture of cells (Weiss and Wardrop, 2010), and does not indicate individual changes in RBC sizes. It is important and timely to study morphometry of RBCs, such as, the relationship between the diameter, the surface area (SA) and the MCV of individual RBCs. Several techniques have been proposed for morphological studies of biological specimens, such as optical coherent tomography and confocal microscopy. The information presented would be valuable for early detection of cellular changes in dogs with subclinicalor clinicaldiseases
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call