Relationship research between expression of HPV L1 capsid protein and cervical lesions degree

Abstract

Objective To investigate the clinical significance of the expression of Ll capsid protein in cervical lesion cells infected with human papillomavirus (HPV), for the prevention of cervical cancer and suggestion of progression and probability of potential lesions. Methods 259 different levels of cervical lesion patients were patients group, including 103 cases negative for intraepithelial lesion and malignancy (NILM), 91 cases with atypical squamous cells of undetermined significance(ASCUS), 36 cases with low-grade squamous intraepithelial lesion (LSIL), 23 cases with high-grade squamous intraepithelial lesion(HSIL), and 6 cases with squamous cell carcinoma (SCC). Cervical cell samples of 50 normal physical examination people were control group. Immunohistochemical staining for Ll capsid protein and PCR-based reverse blot hybridization for 23 HPV DNA were employed. Results Both Ll capsid protein detection and PCR -based reverse blot hybridization detection were negative in the control group.The infection ratio of HPV were 23.3%, 69.2%, 83.3%, 87.0% and 100% in woman with NILM, ASCUS, LSIL, HSIL and SCC, respectively. The constituent ratio of high-risk HPV (HR-HPV)were 79.2%, 92.1%, 96.7%, 100% and 100% in HPV positive patients with NILM, ASCUS, LSIL, HSIL and SCC, respectively. The positive rates of HPV Ll capsid protein in NILM, ASCUS, LSIL, HSIL and SCC were 25.2%, 31.9%, 63.8%, 17.4% and 0.0%, respectively. Conclusions Both infection ratio of HPV and constituent ratio of HR-HPV were increased gradually with the aggravation of cervical lesions, and the infection ratio of HPV and constituent ratio of HR-HPV were closely related to the degree of cervical diseases. The detection results of Ll capsid protein were on the rise in the early phase of cervical lesion, however, declining in the late phase of cervical lesion, the expression of HPV Ll capsid protein associates with the degrees of cervical lesion, which may serve as an indicator of cervical precancerous lesions of the follow-up. Key words: Papillomavirus, human; Ll capsid protein; Immunohistochemical staining; Cervical cancer