Study of clinical application of HPV L1 capsid protein combined with HPV typing and TCT detection in diagnosis and treatment of cervical lesion

Abstract

Objective To investigate the expression of human papilloma virus L1 (HPV L1)capsid protein in cervical lesions and different human papillomavirus (HPV) subtypes, and to guide clinical triage management and best individual treatment. Methods Retrospective analysis of 2012 January to 2014 January in Jiaxing Hospital of Traditional Chinese Medicine gynecology clinic for HPV L1 protein combined with HPV type, and liquid-based cytology test (TCT) of 176 patients data. Results The positive expression rate of HPV L1 protein with TCT examination in the negative for intraepithelial lesion or malignancy (NILM), atypical squamous cells of undetermined significance(ASCUS), low-grade squamous intraepithe lial lesion (LSIL), atypical squamous cells not except high lesion (ASC-H), high-grade squamous intraepithelial lesion (HSIL), and squamous-cell carcinoma (SCC) was 28.99%, 44.19%, 64.44%, 22.22%, 12.50%, and 0, respectively. No significant differences were found between the NILM and ASCUS groups (P>0.05). The positive expression rate of HPV L1 protein in LSIL group was the highest, and it was statistically significantly different from ASC-H and HSIL groups (χ2=3.88, 5.50, P 0.05); and statistically significant difference was found between CIN Ⅰ group and CIN Ⅱ, CIN Ⅲ group (χ2=4.53, 5.56, P 0.05). Conclusions Detection of HPV L1 protein is of clinical value to evaluate the risk of cervical lesions. HPV L1 protein combined with HPV type and TCT detection is helpful for traffic management and personalized treatment, and benefit patients with cervical lesions. Key words: Papillomaviridae/ME; Viral envelope proteins/ME; Cytological techniques; Uterine cervical diseases/ME/DI/TH