RELATIONSHIP OF THE LEVELS OF TRYPTOFAN METABOLITES WITH ADIPOKINES AND MYOKINES IN PATIENTS WITH VARIOUS OBESITY PHENOTYPES

Abstract

Relevance. In the last 10 years, there has been an active study of the system of conjugation of the metabolism of a macroorganism and its microbiome. The microbiotic conversion of tryptophan into biologically active signaling molecules is a potential regulatory mechanism by which the intestinal microbiota can change the metabolism of both intestinal cells and the entire macroorganism. However, the content of tryptophan metabolism metabolites in patients with metabolically healthy (MHO) and metabolically unhealthy obesity (MUHO), as well as the relationship of these metabolites with adipokines and myokines, has not yet been studied. Purpose of the study. To study the content of metabolites of tryptophan metabolism in the blood serum of obese patients and to evaluate the relationship between the content of adipokines and myokines and the content of tryptophan metabolism metabolites of bacterial and non-bacterial origin in the blood serum of patients with MSO and MNSO. Material and methods. 266 patients were examined, including 138 healthy non-obese volunteers and 128 obese patients, of whom two subgroups were formed: 30 patients with MHO and 41 patients with MUHO. Metabolite concentrations in blood and feces were determined using high performance liquid chromatography. Quantitative analysis of adipokines and myokines was performed by multiplex enzyme immunoassay. The content of metabolites of tryptophan metabolism in blood serum was assessed by high performance liquid chromatography with mass spectrometric detection. Results. For patients with obesity, an increase in the serum concentration of kynurenine, kynurenic and quinoline acids, indole-3-lactate, indole-3-butyrate and indole-3-acetate was established. Patients with MHO and MUHO were statistically significantly different only in terms of serum tryptamine concentration. Regardless of the presence/absence of metabolic disorders in obese patients, it has been established that xanthurenic and quinoline acids are interrelated with the concentration of myostatin in the blood serum. At the same time, for patients with MHO, it was shown that the serum concentration of the dominant catabolite of the indole pathway, indole-3-acetate, is interconnected with the content of insulin and leptin in the blood serum. Whereas in patients with MUHO, leptinemia is consistent with a high concentration of anthranilic acid in the blood serum, and hyperinsulinemia, on the contrary, is associated with a low concentration of indole-3-propionate in the blood serum