Relationship of retinol binding protein 4 and high sensitive C-reactive protein with diabetic retinopathy

Abstract

Objective To detect serum retinol binding protein 4 (RBP4) and high-sensitivity C-reactive protein (hs-CRP) in patients with diabetic retinopathy (DR), and to explore the relationship of RBP4 and hs-CRP with DR. Methods A total of 123 patients with type 2 diabetes hospitalized in the Department of Endocrinology in Baoding NO.1 Central Hospital were selected as research subjects, including 41 cases of type 2 diabetes mellitus (DM group), 40 cases of non-proliferative diabetic retinopathy (NPDR group), Forty cases of proliferative diabetic retinopathy (PDR group). Forty cases of normal person were selected as control (NC group). Fasting blood glucose, tiglycerides, total cholesterol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, uric acid and homocyseine were measured by Hitachi 7600 automatic biochemical analyzer. Fasting insulin were measured by electrochemical luminescence, and homeostasis model assessment-insulin resistance(HOMA-IR) was calculated. RBP4 and hs-CRP were measured by enzyme linked immunosorbent assay. The relationship among the indicators mentioned above was analyzed by Pearson correlation analysis, and Logistic stepwise regression analysis was used to analyze the risk factors of DR. Results Compared with NC group, RBP4, hs-CRP in DM group, NPDR group and PDR group were significantly increased; compared with NPDR group, RBP4 and hs-CRP were significantly higher in PDR group(F=33.224, 597.296, all P<0.05). RBP4 was positively correlated with systolic pressure, diastolic blood pressure, fasting blood glucose, HOMA-IR, tiglycerides, total cholesterol, homocyseine and hs-CRP(r=0.172-0.900, all P<0.05), but was negatively correlated with high density lipoprotein-cholesterol(r=-0.406, P<0.05). Logistic stepwise regression analysis showed that systolic pressure(OR=3.947, 95%CI: 1.484-10.502, P=0.006), fasting blood glucose(OR=43.494, 95%CI: 10.490-180.325, P=0.000), RBP4 (OR=2.183, 95%CI: 1.043-4.570, P=0.038) and hs-CRP(OR=6.802, 95%CI: 1.791-25.833, P=0.005) were risk factors of DR. Conclusion RBP4 and hs-CRP are significantly increased in patients with DR, and are related to the occurrence and development of DR. Key words: Retinol binding protein 4; High-sensitivity C-reactive protein; Type 2 diabetes mellitus; Diabetic retinopathy