Abstract
Abstract : Earlier observations revealed that incubation of media and the attendant changes in oxidation-reduction potential (ORP) were related to improved cell production. This is a report of work done to show that the higher levels and increased rates of growth of cells grown in incubated medium are associated with the ORP level of the medium before its inoculation with cells. Work was done using 250-ml centrifuge spinner bottles as the culture vessels. Further work is needed to establish the desirability of deliberate poising of media prior to use for studies in small vessels and flasks.
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