RELATIONSHIP OF MYOSTATIN GENE POLYMORPHISM WITH SOME GROWTH TRAITS OF COMMON CARP CYPRINUS CARPIO L.

Al-Khshali & Saleh

doi:10.36103/ijas.v51i1.930

Abstract

The present study was aimed to investigate the polymorphism of the myostatin gene and its relation with some growth traits,included feed conversion rate and efficiency, protein intake and protein efficiency ratio in 68 specimens of common carp Cyprinus carpio. Sequencing of single nucleotide polymorphism (SNP) showed three genotypes at site T2230C in the myostatin gene. The distribution rates were 5.88, 38.24 and 55.88% for TT, TC and CC respectively, and the variation among them was high significant, the allelic frequency was 0.25 for allele T, while it was 0.75 for C. Effect of the genotype of the myostatin gene was significantly in the feed conversion rate and efficiency, the protein intake and the protein efficiency ratio of the common carp with the variation of the genotypes of myostatin gene, whereas the feed conversion rate was 6.18 , 6.00 and 4.50 at TC , TT and CC respectively. Based on the mentioned results , the positive relation between myostatin gene polymorphism with some growth traits observed in this study may be a useful biomarker in the selection and crossing the genotypes that have achieved the best performance in common carp .

Highlights

Myostatin gene is called the Growth Differentiation Factor GDF-8 (MSTN), with size of 6.4 kilo bite (Kg) [4],it is located on chromosome 11 in common carp and consists of three exons and two introns [20]
Myostatin gene is associated with some of traits related to general animal performance such as feed conversion ratio, chest muscle depth, carcass weight without intestines, blood oxygen level, antibody system in poultry [3], weight gain and meat production in cattle [10], and is associated with most traits including body weight, total length and body height in fish [15], body weight and condition factor in common carp Cyprinus carpio L. [1,20], body weight and the fillets weight of Atlantic salmon Salmo salar [13], as well as the body weight in tilapia Oreochromis niloticus
Many genetic markers are currently being used, such as single nucleotide polymorphism (SNP) and Quantitative Loci (QTL), which have helped detect the structure of the myostatin gene and the relationship between its polymorphism and some growth traits in some commercial fish species such as tilapia and some genetically improved salmon species

Summary

INTRODUCTION

Myostatin gene is called the Growth Differentiation Factor GDF-8 (MSTN), with size of 6.4 kilo bite (Kg) [4],it is located on chromosome 11 in common carp and consists of three exons and two introns [20]. The parts of the genes studied have been verified by electronic genome browsers: (NCBI) National Centre for Biotechnology Information Forward: 5'AGCCTACCATAAAAGGTGTGTG-3', Reverse: 5' TCAATAGTGTCCATTCCCAAGT-3' Growth traits studied for a period of 82 days were : 1- Feed Conversion Rate (F.C.R) Which is one of the criteria for measuring the efficiency of the diet, and represents the ratio between feed provided to fish, to the wet increase in fish weight [16], and was calculated by the formula mentioned by Alazzawy and Alkhshali [1]: F.C.R. Consumed in the diet, and was estimated L) by oxygen meter (Hana 208), and pH by pH according to the equation mentioned by meter (Hana 196). The following law was applied to calculate the allele frequency in each mutation according to Hardy Weinberg's equilibrium

RESULTS AND DISCUSSION

Polymorphisms of the myostatin gene by the sequence of nitrogen bases

Qi square

Biophysical Research Communications