Abstract
ABSTRACT Myostatin (MSTN) is a negative regulator of skeletal muscle growth. In order to investigate whether there is a correlation between MSTN polymorphisms and chicken production performance, in this study, single nucleotide polymorphisms (SNPs) in MSTN gene were examined across 180 Daheng broilers by direct sequencing of PCR product, and the correlations between the genotype and body weight at the age of 1-10 weeks and carcass traits at the age of 73 day were analyzed. Five SNPs (rs313622770, rs313744840, rs316247861, rs314431084, rs317126751) of MSTN gene were identified across Daheng broiler samples, and four haplotypes were reconstructed based on the five SNPs. Results of association analysis showed that four (rs313622770, rs313744840, rs316247861 and rs317126751) of these SNPs had significant association with some growth traits (p<0.05), but there were no significant effect on carcass traits and the four SNPs were strong linkage. For rs314431084, there was no significant correlation between different genotypes and growth or carcass traits. The AA genotype of rs313622770, GG genotype of rs313744840, CC genotype of rs316247861, TT genotype of rs317126751 were good for chicken growth. Diplotypes were significantly associated with chest muscle and leg muscle weight (p<0.05). Overall, these results provide evidence that polymorphisms in MSTN gene are associated with growth traits in chicken. The SNPs in MSTN gene could be utilized as potential markers for marker-assisted selection (MAS) during chicken breeding.
Highlights
Meat production is one of the most important economic traits in chicken, and how to improve meat production is one of the most important objectives of breeding researchers
polymorphism information content (PIC) test results indicate that SNP1, SNP2, SNP3 and SNP5were intermediate polymorphism
Myostatin is a negative regulator of skeletal muscle Zhang et al (2012) found SNP4 was significantly growth, and loss of myostatin function will lead to a associated with body weight in Bian chicken, the dramatic and specific increase in skeletal muscle mass genotypes AA and GA had significantly higher body eRBCA-2018-0808
Summary
Meat production is one of the most important economic traits in chicken, and how to improve meat production is one of the most important objectives of breeding researchers. Researchers have selected lots of candidate genes associated with growth traits, Myostatin (MSTN) is one of these genes identified as a negative regulation factor of skeletal muscle growth (Wehling et al, 2000). MSTN, known as growth differentiation factor 8 (GDF-8), is a member of the transforming growth factor beta (TGF-β) family. It has been widely investigated in livestock, poultry, rodents and humans (Schiffer et al, 2011; Varga et al, 2003; Wang et al, 2014). A number of evidence has shown that MSTN acts as a negative regulator of skeletal muscle growth, and loss or decrease of its activity will cause excessive development of animal muscle (Clop et al, 2006). MSTN controls embryonic myoblast proliferation to regulate skeletal muscle size, Kocamis et al (1999) investigated the developmental pattern of MSTN gene in chicken embryonic development and found that the expression of MSTN gene has been detected as early as the blastoderm stage, and they suggested MSTN gene eRBCA-2018-0808
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